Table 2.
Primers used in RT‐qPCR
| Primers | Sequence (direction 5′ > 3′) | Reference |
|---|---|---|
| TNF‐α | ||
| Forward | TCCAGCTGACTAAACATCCT | Oben et al. (2010) |
| Reverse | CCCTTCATCTTCCTCCTTAT | |
| IL‐6 | ||
| Forward | TTCACAGAGGATACCACTCC | Oben et al. (2010) |
| Reverse | GTTTGGTAGCATCCATCATT | |
| IL‐1β | ||
| Forward | CTTTGAAGTTGACGGACCC | Mandrekar, Ambade, Lim, Szabo, and Catalano (2011) |
| Reverse | TGAGTGATACTGCCTGCCTG | |
| αSMA | ||
| Forward | ATCTGGCACCACTCTTTCTA | Oben et al. (2010) |
| Reverse | GTACGTCCAGAGGCATAGAG | |
| Col1α2 | ||
| Forward | GAACGGTCCACGATTGCATG | Oben et al. (2010) |
| Reverse | GGCATGTTGCTAGGCACGAAG | |
| Col3α1 | ||
| Forward | CTGGTTTCTTCTCACCCTTC | This study |
| Reverse | GGCTTCCAGACATCTCTAGA | |
| MCP1 | ||
| Forward | CAGGTCCCTGTCATGCTTCT | This study |
| Reverse | GCTTCTTTGGGACACCTGCT | |
| GAPDH | ||
| Forward | GTATGTCGTGGAGTCTACTG | This study |
| Reverse | GGTGCAGGATGCATTGCTGA | |