Table 6.
Immunohistochemistrical‐histomorphometrical analysis of hepatic tissues from CCl4‐treated mice
| Items (Unit) Groups | Positive cells by immunohistochemistry (cells/1000 hepatocytes) | |||
|---|---|---|---|---|
| Cleaved caspase‐3 | Cleaved Poly(ADP‐ribose) polymerase | Nitrotyrosine | 4‐Hydroxynonenal | |
| Controls | ||||
| Intact | 2.00 ± 1.41 | 4.10 ± 2.88 | 17.20 ± 10.12 | 15.80 ± 8.38 |
| CCl4 | 718.20 ± 108.68a | 714.40 ± 111.79c | 707.10 ± 101.38c | 757.10 ± 120.07c |
| Silymarin | 383.00 ± 104.97ab | 288.80 ± 64.00cd | 331.50 ± 115.94cd | 440.20 ± 135.73cd |
| BH extracts | ||||
| BHw | 488.90 ± 119.27ab | 470.40 ± 126.21cd | 486.90 ± 108.44cd | 535.70 ± 107.30cd |
| BHj | 433.20 ± 107.51ab | 454.80 ± 96.07cd | 442.10 ± 66.79cd | 480.90 ± 112.66cd |
| BHe | 310.10 ± 111.16ab | 196.90 ± 62.99cd | 291.60 ± 69.14cd | 264.10 ± 70.92cd |
Values are expressed mean ± SD of 10 mice. BH: Blue honeysuckle (Berries of Lonicera caerulea var. edulis L., Caprifoliaceae); BHw: lyophilized powder of BH aqueous extract; BHj: lyophilized powder of BH squeezed juice; BHe: lyophilized powder of BH enzyme extract.
a p < 0.01 as compared with intact vehicle control by LSD test. b p < 0.01 as compared with CCl4 control by LSD test. c p < 0.01 as compared with intact vehicle control by MW test. d p < 0.01 as compared with CCl4 control by MW test.