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. 2019 Jan 21;10:342. doi: 10.1038/s41467-018-08178-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cryo-electron tomography reveal F-actin organization in iTNTs. a Segmentation rendering obtained from a tomogram showing iTNTs (Supplementary Movie 8). The average size and spacing between filaments of an iTNT shown in b were measured by computing the plot profile of actin bundles residing within. c–e Filament-to-filament distance measured by extracting peaks in the frequency domain using the fast Fourier transform (white arrowheads, e) and by computing plot profiles (c). f Model of the actin filaments displaying a parallel bundle in iTNTs obtained by retransforming peaks in the frequency domain shown in e. Insets in b and f show distances between the average measurements in nm obtained by the analysis shown in c and d. g High-magnification cryo-tomogram slice of two iTNTs (Fig. 3b and Supplementary Movie 4). h Cross-section of the cryo-electron tomogram in g displaying the actin arrangement within two iTNTs. Scale bars: a, 100 nm; b, f, 20 nm; g, h, 100 nm