FIGURE 2.

Il33^−/− mice exhibit impaired virus-specific CD8⁺ memory T cell inflation during chronic MCMV infection. C57BL/6 (WT) and Il33^−/− mice were infected with MCMV. (A) Frequencies of tetramer-binding CD8⁺ T cells specific for M45, m139, M38, or IE3 were quantified among leukocytes isolated from spleens, lungs, and salivary glands on day 60 postinfection (pi). Horizontal bars depict mean percentage of viable CD3⁺CD8⁺ T cells (n = 9 mice per group). (B) Total numbers of tetramer-binding CD8⁺ T cells specific for M45, m139, M38, or IE3 were quantified among leukocytes isolated from spleens, lungs, and salivary glands on day 60 pi. Data are shown as mean ± SEM (n = 12–16 mice per group). (C) Total numbers of naive (T_N) and memory T (T_EM and T_CM) cells within the CD4⁺ and CD8⁺ compartments were quantified among leukocytes isolated from spleens, lungs, and salivary glands on day 60 pi. Data are shown as mean ± SEM (n = 9–12 mice per group). Results are concatenated from three independent experiments (A–C). *p ≤ 0.05, ***p ≤ 0.001.