Figure 8.

(Left) Human iN populations robustly express MAP2 in 2D and 3D conditions, while populations of unconverted, proliferative Ki67-expressing iPS cells persist in iN populations plated in 2D conditions. Scale bar, 100 mm (Carlson et al., 2016). (Right) snapshots of representative fields of neuronal cultures grown on 2D-PDMS (top) and 3D-PDMS (bottom) substrates, stained with the Oregon Green 488-BAPTA-1 AM. Scale bar: 50 μm. Repetitive Ca²⁺⁻events spontaneously recorded in hippocampal cultures of 9 DIV highlighted an higher frequency and synchronization of events in 3D cultures (Bosi et al., 2015).