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. 2018 Nov 16;116(1):127–141. doi: 10.1016/j.bpj.2018.11.011

Figure 2.

Figure 2

ICM organoids mimic the mouse ICM. Mouse tet::GATA4 ESCs were stimulated for 6 h with doxycycline (+ dox) to induce PrE differentiation. Cells that were not stimulated with dox (− dox) served as control. (A) After removal of dox, GATA6 and NANOG were coexpressed in cells of the two-dimensional monolayer of mESCs (see box for a magnified section of a coexpressing cell). (B) 24 h after ICM organoid formation, GATA6 and NANOG were mutually exclusively expressed within the ICM organoids. (C) After 48 h, GATA6-positive cells arranged at the rim of the ICM organoids. (D) After 72 h, NANOG was downregulated. Images show a single slice from the aggregate’s center. Microscope: Zeiss LSM780; objective: 63×/1.40 oil; scale bars, 20 μm. To see this figure in color, go online.