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. 2018 Aug 13;115(2):343–356. doi: 10.1093/cvr/cvy207

Figure 6.

Figure 6

Transplantation of Y-27632 or verapamil-preconditioned hiPSC-CMs promotes angiogenesis in MI mice. (A and B) Tissue sections from the border-zone of MI hearts obtained from mice that received intramyocardial injection of (A) PBS-, hiPSC-CMs−RI-, and hiPSC-CMs+RI, or (B) PBS-, hiPSC-CM−VER, and hiPSC-CM+VER were immunofluorescently stained for the expression of CD31 and cTnT. Sections from the corresponding regions of the hearts from Sham mice were used as control. Vessel density was determined as the number of CD31-positive vascular structures per square millimetre. Bar = 20 µm. n = 5 mice per treatment group; four sections per mice were evaluated. *P < 0.05 vs. Sham mice; †P < 0.05 vs. MI mice. (C and D) Cell adhesion (VCAM1), pro-angiogenic (VEGF, FGF), pro-migratory (G-CSF), and pro-apoptotic (TNF-α) cytokines released from (C) hiPC-CM+RI or (D) hiPC-CM+VERin vitro were determined by ELISA. n = 3 replicates per group.* P < 0.05 vs. –RI group. One-way ANOVA analysis with Bonferroni correction was performed (A and B). Unpaired Student’s t-test was performed (C and D). Data were presented as the mean ± SEM.