Figure 2.

Relationships between the change in plasma FGF21 concentration after 24-h low-protein high-fat overfeeding and the percentage change in 24-h EE from energy balance (A) and free-living weight change after 6 months (B). The macronutrient composition of LowPro/HighFat overfeeding diet was: 51% carbohydrate, 46% fat, and 3% protein. The percentage change in 24-h EE was calculated as: (24-h EE_{overfeeding diet} − 24-h EE_{energy balance})/24-h EE_{energy balance} × 100. One subject found to have impaired fasting glucose on the OGTT and one subject with benign glycosuria were excluded from these analyses because these conditions are known to affect 24-h EE. Associations were quantified by the Pearson correlation index.