Skip to main content
. 2018 Nov 26;294(3):805–815. doi: 10.1074/jbc.RA118.004964

Figure 2.

Figure 2.

SZMP is not required for AICAR toxicity but rather contributes to its detoxification in yeast. A–C, deletion of the ADE13 gene leads to increased ZMP accumulation and AICAR toxicity. A, yeast strains (Y2950 and Y10422) were grown overnight, serially diluted (1/10), and spotted on SDcasaWAU medium containing or lacking AICAR. The plates were imaged after 48 h of incubation at 30 °C. B and C, ZMP (B) and SZMP (C) relative content was determined on metabolic extracts from three independent cell cultures of each strain (Y2950 and Y10422) grown in SDcasaWAU medium and treated with AICAR (0.5 mm) for 24 h. Relative contents were set at 1 for the ADE13 control strain. D–G, favoring SZMP synthesis from ZMP lowers AICAR toxicity. D–F, metabolic analyses were done on three independent extractions from cells (Y2950 and Y3655) grown in SDcasaWAU medium and treated with AICAR (0.5 mm, 24 h). Relative content was set at 1 for the FUM1 control strain (Y2950). G, AICAR resistance was increased in a FUM1-deleted strain (Y3655) grown for 48 h in SDcasaWAU medium at 30 °C. B–F, errors bars and statistics correspond to standard deviation and Welch's unpaired t tests. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; UD, undetectable.