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. 2018 Nov 30;294(3):932–940. doi: 10.1074/jbc.RA118.005568

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© 2019 Zhong et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — Tol2-mediated enhancer trap is combined with Cre/loxP for the screen of pancreatic β cell– and liver-specific genes. A, Cre/loxP–based transgenic lines Tg(fabp10:loxP-CFPNTR-loxP-DsRed; 10×UAS:Cre, cryaa:Venus) and Tg(ins:loxP-CFPNTR-loxP-DsRed; 10×UAS:Cre, cryaa:Venus) were constructed as schematically illustrated. The expression of Venus in the eyes indicates the 10×UAS:Cre transgene in the genome. B, schema showing the procedure for the screen. Cre/loxP–based transgenic reporter lines for pancreatic β cells (left) and hepatocytes (right) were crossed with Tol2-mediated F₀ enhancer trap line, respectively. The F₁ larvae with red fluorescence in the β cells or hepatocytes were selected for genomic identification. F₁ larvae without red color in targeted organs were subjected to further regeneration studies.