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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: ACS Chem Biol. 2016 Dec 23;12(2):435–443. doi: 10.1021/acschembio.6b00945

Figure 3.

Figure 3.

Dicer cleavage assays of full length 5′-AlexaFluor 647-labeled pre-miR-21. (A) In-gel fluorescence of 5′-AlexaFluor 647-labeled premiR-21 cleaved with Dicer in the presence of increasing concentration of 1. Arrow indicates the band corresponding to the unprocessed premiR-21, which was used for quantitation. (B) Quantification of in-gel fluorescence of 5′-AlexaFluor647-labeled pre-miR-21 in the presence of Dicer and the miR-21 binding compounds at a concentration of 1 μM. Treatments were performed in triplicate, and compound treatment was normalized to DMSO controls with and without Dicer within the same experiment. The error bars indicate the standard deviation determined from the three independent experiments.