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. Author manuscript; available in PMC: 2019 Apr 5.
Published in final edited form as: Science. 2018 Aug 23;362(6410):eaas8843. doi: 10.1126/science.aas8843

Fig. 5. Functional characterization of HH signal biogenesis.

Fig. 5

(A) Location of the key residues for HH signal biogenesis. (B) Mutation on either interface or palmitoylation site of SHH-N abolishes HH signaling. HH activity was measured by luciferase assay. Data are mean ± s.d. (n = 3). SHH-N in conditioned medium was detected by western blotting. (C) HH signaling in Ptch1−/−MEFs transfected with PTCH1 and treated with SHH-N ligand. Luciferase activity measured as described in Methods section. Data are mean ± s.d. (n = 3).