Fig. 2.

Functional annotation of down-regulated genes and anti-HIV activity of up-regulated genes. a Gene Ontology of down-regulated DEGs in MADNT. b KEGG pathway enrichment of down-regulated DEGs in MADNT. c Validation of anti-HIV activity of night selected up-regulated DEGs. HEK293T cells were transfected with pNL4-3luc.R-E-, VSV-G with myc tagged-CMPK1, METTL9, GADD45A, CHPT1, OAT, PPKAR2B, ZNF395, SPOCK2, CBX7 or empty vector or control gene (GAD45G and SLFN11), 48 h post-transfection, virions in the supernatant were filtered and used for infecting SupT1 and Jurkat cells. 48 h post-infection, luciferase activity in SupT1 cells (upper panel) and Jurkat cells (lower panel) were measured. Black columns represent controls and white columns represent DEGs. EV denotes empty vector. d Gag expression influenced by these up-regulated DEGs. The expression of HIV Gag, myc-tagged DEGs and β-actin were determined by Western blotting. e Venn. Diagram of DEGs from CD8+ and CD4+ T cells of ECs and all NPs