Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 22;18:15. doi: 10.1186/s12943-019-0942-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Overexpression of OTUD6B-AS1 markedly suppressed the proliferation of ccRCC cells in vitro. a OTUD6B-AS1 expression levels in the ccRCC cell lines (786-O, Caki-1769-P, OS-RC-2 and ACHN) compared with that in the human renal tubular epithelial cell line (HK-2). b ACHN and OS-RC-2 cells treated with 5 μM 5-aza-CdR. c qRT-PCR analysis of the OTUD6B-AS1 expression in the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. (d, e) MTT cell proliferation assays performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. (f, g) Colony formation assays performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. h Cell immunofluorescence staining assay performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. *P < 0.05, **P < 0.01, *** P < 0.001