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. 2019 Jan 22;18:15. doi: 10.1186/s12943-019-0942-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — OTUD6B-AS1 overexpression inhibited the migration and invasion of ccRCC cells in vitro. (a, b) Transwell assays with Matrigel for cell invasion with the ACHN and OS-RC-2 cell lines transfected with plvx-OTUD6B-AS1 or the empty vector; c western blot analysis of MMP9 expression in cells transfected with plvx-OTUD6B-AS1 or the empty vector. β-Actin internal control. d, e Transwell assays without Matrigel for cell migration with the ACHN and OS-RC-2 cell lines transfected with plvx-OTUD6B-AS1 or the empty vector. The data represent the mean ± SD of 3 replicates. *Indicates a significant difference between the empty vector group and the plvx-OTUD6B-AS1 group. * P < 0.05, **P < 0.01