Fig. 7.

Putative CHH receptors and their tissue distribution. a Pruned tree of CHHRs and amino acid sequence arrangement of putative CHHRs. Transmembrane domains of both Gl_GPCRA9 and Gl_GPCRA12 were predicted using TMHMM online tool. b RT-PCR was carried out using cDNA from ten different organs of G. lateralis. Primers were designed to amplify two putative CHHR receptors (Gl_GPCRA9 and Gl_GPCRA12) (Table 1). Tissue expression pattern obtained from RT-PCR gel image visualized under UV light