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. 2019 Jan 22;10:37. doi: 10.1186/s13287-019-1143-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Catalpol promotes the osteogenic differentiation of BMSCs in vitro. a Chemical structure of catalpol. b Cell viability after catalpol treatment was determined using the CCK-8 assay. c–f Osteogenic differentiation was assessed by ALP staining (c), ALP activity assays (d) and Alizarin Red staining (e). Calcium deposition was determined by measuring optical density (f). The data were confirmed by three repeated tests. The data are presented as the means ± SD. CA, catalpol. *P < 0.05 compared with the control group, ^#P < 0.05 compared with the 10 μM catalpol treatment group