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. 2019 Jan 22;10:38. doi: 10.1186/s13287-019-1141-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Time course assay of 3T3-L1 adipogenesis. The representative fields of phase-contrast (top row), nuclei and lipid droplets detection (middle row), and histograms from a whole well (bottom row) are shown for the five samples: undifferentiated (a), day 2 (b), day 4 (c), day 7 (d), and day 10 (e). Scale bars 100 μm. f The left graph shows average fluorescence measurements of Nile Red staining by spectroscopy (blue bars; left y-axis; n = 8) and average adipogenic scores (orange bars; right y-axis; n = 8–16) for each time points. The right graph indicates correlation between the spectroscopic fluorescence measurements (standard) and adipogenic scores (by FATS). Results are presented as mean ± standard error of mean (SEM)