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. 2019 Jan 22;19:20. doi: 10.1186/s12935-019-0734-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — PXN knockdown attenuated the oncogenic roles of PXN-AS1-L in NSCLC. a The expressions of PXN in PXN-AS1-L stably overexpressed and control A549 cells after transient transfection of PXN specific shRNA were detected by qPCR. b Cell viability of PXN-AS1-L stably overexpressed and control A549 cells after transient transfection of PXN specific shRNA was detected by Glo cell viability assays. c Cell proliferation of PXN-AS1-L stably overexpressed and control A549 cells after transient transfection of PXN specific shRNA was detected by EdU incorporation assays. The red color indicates EdU-positive cells. Scale bars = 200 μm. d Cell apoptosis of PXN-AS1-L stably overexpressed and control A549 cells after transient transfection of PXN specific shRNA was detected by TUNEL assays. e Cell migration of PXN-AS1-L stably overexpressed and control A549 cells after transient transfection of PXN specific shRNA was detected by transwell assays. Scale bars = 100 μm. Results are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 by Student’s t-test