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. 2018 Dec 11;17(2):2257–2265. doi: 10.3892/ol.2018.9822

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-744 downregulation inhibits cell proliferation. (A) miR-744 knockdown significantly decreased PC3 cell proliferation as determined by the Ki-67 immunofluorescence assay (magnification ×200). (B) Cell cycle profiles analyzed by flow cytometry. (C) Alterations in cyclin-dependent kinase 4, cyclin D1, and proliferating cell nuclear antigen protein levels detected by western blotting. (D) The protein level of p21 was increased upon miR-744 knockdown. *P≤0.05 vs. respective NC (n=3 independent experiments for each condition). miR, microRNA; CDK4, cyclin-dependent kinase 4; PCNA, proliferating cell nuclear antigen; p21, cyclin-dependent kinase inhibitor p21; NC, negative control.