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Primers used for reverse transcriptase-quantitative polymerase chain reaction.
| Gene | Primer sequence (5′→3′) | Amplicon size (base pairs) |
|---|---|---|
| CD146 | F: AGCTCCGCGTCTACAAAGC | 102 |
| R: CTACACAGGTAGCGACCTCC | ||
| VEGFA | F: AGGGCAGAATCATCACGAAGT | 75 |
| R: AGGGTCTCGATTGGATGGCA | ||
| GAPDH | F: GGAGCGAGATCCCTCCAAAAT | 197 |
| R: GGCTGTTGTCATACTTCTCATGG |
F, forward primer; R, reverse primer; CD, cluster of differentiation; VEGFA, vascular endothelial growth factor A.
