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. 2019 Jan 21;19:20. doi: 10.1186/s12866-019-1387-9

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of mutation of lolA on the expression of genes encoding extracellular enzymes or products associated with attachment. The expression level of extracellular enzyme genes (engA, manA, pelA, and prt1) and attachment-related genes (galE, ha, xanA, and yapH) in the wild-type strain Xcc17 (black bar) and lolA mutant strain H27 (white bar) was evaluated through RT-qPCR. The gene ID is based on X. campestris pv. campestris strain 8004 and listed in parentheses. The relative expression level of each gene in Xcc17 and H27 was normalized to its 16S rRNA content. Values presented are the mean ± standard deviation (n = 3). Significance was determined using the Student t test (* indicates significance at p < 0.05)