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. 2018 Dec 24;20(1):69. doi: 10.3390/ijms20010069

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cleavage of ssDNAs containing clustered AP site analogues. The ssDNAs (100 nM) containing (a) consecutive Spacer C12 or 18, (b) tandem Spacers C12 and 18, or (c) tandem Spacer 18 separated by four normal bases or (d) a different middle AP site analogue were incubated with TeuendoIV (5 nM) at 55 °C for the indicated time. The combinations of AP site analogues are listed on the top of each panel. Uppercase letter M denotes oligonucleotides marker. The cleavage percentages of substrates are listed at the bottom of each panel. The symbol of black asterisk and the red letters X, Y and Z denote the fluorescein (6-FAM) group at the 5′-end and the AP-sites, respectively.