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. 2018 Dec 24;20(1):69. doi: 10.3390/ijms20010069

Figure 8.

Figure 8

Cleavage reactions of wt (wild type) and mutant TeuendoIV. Mutations on key conserved residues potentially involved in (a) coordinating metal ion cofactors, (b) penetrating into the duplex minor groove, and binding the (c) AP site-containing strand and (d) complementary strand were used to assay AP endonuclease activities (5 nM enzymes) on ssDNA and dsDNA with an internal dSpacer (100 nM) at 55 °C for the indicated time. The cleavage percentages of substrates are listed at the bottom of each panel. The symbol of black asterisk and the red letter X denote the fluorescein (6-FAM) group at the 5′-end and the AP-site, respectively.