Figure 7.

Pemetrexed triggers endoplasmic reticulum stress in human esophageal squamous cell carcinoma cells. (A) Eca-109 and (B) EC9706 cells were treated with 0, 2.5, 5 or 10 µM pemetrexed and incubated for 36 h. Following treatment, the protein expression of IRE1α, Bip and CHOP was quantified via western blot analysis. All data are presented as the mean ± standard deviation, *P<0.05, **P<0.01, ***P<0.001. NS, not significant; PEM, pemetrexed; IRE1α, inositol-requiring enzyme 1α; CHOP, CCAAT-enhancer-binding protein homologous protein; Bip, binding immunoglobulin protein.