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Effects of ZO-1 on cell cycle arrest of LC cells in vitro. (A) Following transfection with ZO-1-overexpression plasmid (ZO-1) or empty plasmid (vector) for 48 h, or untransfected (CON), the cell cycle distribution of HepG2 cells was determined by propidium iodide staining and flow cytometry. (B) ZO-1 overexpression caused a significant accumulation of cells in G0/G1 phase and a marked decrease in S-G2 phase compared with empty-vector-transfected cells. *P<0.05.
