Effects of Beclin 1 expression on phenotype-associated molecules of HCT-15 and HCT-116 cells. Reverse transcription-quantitative polymerase chain reaction analysis of (A) HCT-15 and (B) HCT-116 cells, and western blot analysis of (C) HCT-15 and (D) HCT-116 cells were employed to determine the expression of phenotype-associated molecules in the control, vector mock and Beclin 1 transfectants. Results are representative of three independent experiments and are expressed as the mean ± standard deviation. *P<0.05 vs. the mock and control groups. JNK, c-Jun N-terminal kinase; Bax, Bcl-2-associated X protein; TAK1, transforming growth factor β-activated kinase 1; CDK4, cyclin-dependent kinase 4; Bcl-2, B-cell lymphoma 2; LC-3B, light chain 3B; C1, clone 1; C2, clone 2.