Figure 1.

Let-7b is downregulated in osteosarcoma cell lines and osteosarcoma tissues. (A) RT-qPCR was used to determine the endogenous levels of Let-7b in human osteosarcoma cell lines U2OS, MG-63 and SAOS-2, and the normal human osteoblastic cell line hFOB 1.19. The expression levels of Let-7b were normalized to that of U6 RNA. *P<0.05, **P<0.01 vs. hFOB 1.19 cells. (B) The expression of Let-7b in a total of 60 paired osteosarcoma tissues and adjacent non-tumor tissues was determined by RT-qPCR. *P<0.05 vs. adjacent tissue. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.