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. 2018 Dec 4;17(2):1646–1654. doi: 10.3892/ol.2018.9793

Figure 2.

Figure 2.

Let-7b inhibits the proliferation of osteosarcoma cells. (A) The reverse transcription-quantitative polymerase chain reaction was used to determine Let-7b expression following transfection with NC or Let-7b mimic in U2OS and SAOS-2 cells. Non-transfected cells were used as a control. MTT assays were performed to determine the proliferation of (B) U2OS and (C) SAOS-2 cells following transfection with NC or Let-7b mimic. Non-transfected cells were used as control. Flow cytometry was used to determine the cell cycle distribution of (D) U2OS and (E) SAOS-2 following transfection with NC or Let-7b mimic. Non-transfected cells were used as a control. *P<0.05, **P<0.01 vs. control. Each sample was analyzed in triplicate. Results are presented as the mean ± standard error of the mean. NC, negative control; OD, optical density.