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. 2018 Dec 4;17(2):1646–1654. doi: 10.3892/ol.2018.9793

Figure 5.

Figure 5.

IGF1R is involved in Let-7b-mediated proliferation and invasion of osteosarcoma cells. (A) Reverse transcription-quantitative polymerase chain reaction and (B) western blot analysis were used to determine the expression of IGF1R in U2OS and SAOS-2 cells transfected with Let-7b mimic or co-transfected with Let-7b mimic and IGF1R plasmid. MTT assays were used to determine the proliferation of (C) U2OS and (D) SAOS-2 cells transfected with Let-7b mimic or co-transfected with Let-7b mimic and IGF1R plasmid. Transwell assays were used to determine the invasion of (E) U2OS and (F) SAOS-2 cells. Results are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control; #P<0.05 vs. Let-7b. IGF1R, insulin-like growth factor 1 receptor; NC, negative control; OD, optical density.