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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: J Neurosci Methods. 2018 Dec 6;312:154–161. doi: 10.1016/j.jneumeth.2018.12.005

Figure 2:

Figure 2:

Transection apparatus for LLSM experiments. (A) Exploded view of the transection tool sitting on the cantilever which in turn sits on the stage. (B) Actual configuration of the transection tool, cantilever and stage for transection. (C) The transection tool in use. In this image, the lid of the heated chamber has been lifted to allow access to the sample. Note that the space around the sample is too small to accommodate a glass pipette mounted on a micromanipulator, even when the lid is open. The transection tool slides along the slots in the back end so that the needle protruding from the front end scratches the glass coverslip in the sample holder at a predictable location. Then, the transection tool is removed and the heated chamber is closed without moving the sample to begin imaging.