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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: J Neurosci Methods. 2018 Dec 6;312:154–161. doi: 10.1016/j.jneumeth.2018.12.005

Figure 3:

Figure 3:

Process for quantifying dystrophy. (A) The 3D-SIM image is converted into a maximum intensity projection. (B) A region of interest and an approximation of the segmentation are defined manually on the projected image. (C) Active contours are used to evolve the approximate segmentation towards the true segmentation. (D) The neutral axis is defined using distance transforms. The distance between the neutral axis and the edge along a line perpendicular to the neutral axis is determined and defined as the local caliber. (E) The standard deviation of the local caliber along the neutral axis is calculated to quantify the degree of dystrophy in the region of interest.