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. 2019 Jan 14;25:409–417. doi: 10.12659/MSM.912042

Figure 3.

Figure 3

Effect of cimifugin on the migration and chemotaxis of RAW264.7 cells. RAW264.7 cells were divided into a control group (no LPS was added), LPS group (treated with 1 μg/mL LPS for 24 h), high-dose group (1 μg/mL LPS+100 mg/L cimifugin for 24 h), medium-dose group (1 μg/mL LPS+50 mg/L cimifugin for 24 h), and low-dose group (1 μg/mL LPS+25 mg/L cimifugin for 24 h). (A, B) Effect of cimifugin on the migration and chemotaxis of RAW264.7 cells after treatment with (A) fetal bovine serum or (B) LPS. Transwell assay was performed to determine the migration of cells. * P<0.05 and ** P<0.01 compared with control group. # P<0.05 compared among indicated groups. (C) Effect of cimifugin on the concentrations of migration- and chemotaxis-associated factors in cell culture supernatants. ELISA was used to determine the concentrations of the factors. * P<0.05 and ** P<0.01 compared with LPS group. # P<0.05 and ## P<0.01 compared among indicated groups.