Figure 8.

The breeding scheme that was used to produce F1 and F3 lineage rats for analysis is shown. Briefly, pregnant F0 dams were treated with a 1 mg/kg/day i.p. injection of either A1221, VIN, or vehicle (VEH: 6% DMSO in sesame oil) from embryonic (E) day 8–18. At the time of treatment, the F1 fetus and the F2 germline are directly exposed. F1 males were mated at PND 90 to colony acclimated breeder females purchased from Harlan. F2 males from the resulting litter were mated to untreated  breeder females at PND 90 resulting in an F3 litter. Only F1 (directly exposed) and F3 (ancestrally exposed) males were used in these experiments.