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. 2018 Nov 13;17(21-22):2496–2516. doi: 10.1080/15384101.2018.1547001

Figure 2.

Figure 2.

FUCCI Cdt130-120 is a phosphomimetic SCFSkp2 sensor. a) Schematics of the first 120 amino acids of Cdt1 and the four different reporter fragments analyzed in this study. Relevant phosphorylation sites are indicated by circles; linker regions derived from expression vectors are gray rectangles. b) Dynamics of the Cdt116-120-mCherry reporter throughout the cell cycle analyzed as in Figure 1(e). Gray lines are 50 randomly selected cells and the magenta line is the mean signal for the population (n = 52). c) Dynamics of the Cdt130-120 (E29A)-mCherry reporter (50 random traces (gray) and mean of the population (magenta), n = 107). d) Dynamics of the Cdt130-120 (E29T)-mCherry reporter (50 random traces (gray) and mean of the population (magenta), n = 73).