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Rottlerin inhibited EZH2 expression at RNA and protein levels. (a) EZH2 mRNA level was measured by RT-PCR in prostate cancer cells with rottlerin treatment. *P < 0.05, **P < 0.01 vs control (DMSO treatment). (b) Top panels, Western blotting analysis were conducted to determine the expression of EZH2 in PC3 and DU145 cells. Bottom panels, Quantitative results are illustrated for Top panel. * P < 0.05, **P < 0.01 vs control (DMSO treatment).
