Figure 5.

In situ hybridization analysis of ZIC2, HUNK, and ANXA10 mRNAs in SSA/Ps and cADNs. ZIC2 expression (red punctate labeling) is (a) present in SSA/Ps and (b) absent in cADNs. HUNK (brown punctate labeling) is (c) almost absent in SSA/Ps (very low-level expression was present at the bottom of serrated crypts, see inset) and (d) expressed in cADNs. Control staining for ANXA10, a known SSA/P-specific marker[25,73–75] was (e) strongly positive in SSA/Ps (dense red punctate labeling) and (f) absent in cADNs.