Figure 1.

In planta phenotypic assay demonstrates similar pycnidia levels of Zymoseptoria tritici isolates on the susceptible wheat cultivar Obelisk. Quantitative differences in (a) necrosis and (b) pycnidia coverage of inoculated leaf areas were manually assessed at 28 days post inoculation based on six symptom levels: 0 (without visible symptoms), 1 (1% to 20%), 2 (21% to 40%), 3 (41% to 60%), 4 (61% to 80%), and 5 (81% to 100%). The three isolates caused different levels of necrosis (two‐sided Mann–Whitney U tests, p ≤ 0.0048), but pycnidia levels were not different (two‐sided Mann–Whitney U tests, p ≥ 0.034)