Table 2.

Zymoseptoria tritici core biotrophic effector candidate genes

Criteria	Significantly up‐regulated at stage B
	Low/no expression during stage C and D
	RPKM stage B > RPKM stage C in Zt05 and Zt09
Expression profiles
Putative functions	Bypass host recognition during biotrophic colonization
Candidate genes	25 (Supporting Information Figure S13)
Candidates encoding hypothetical proteins	23 (Supporting Information Table S15)
Candidates with predicted function	Gene	Function/Functional prediction	PFAM domains	GO terms associated
	MgNLP (Motteram et al., 2009) Zt09_chr_13_00229	Necrosis and ethylene‐inducing peptide 1‐like protein MgNLP (Kettles, Bayon, Canning, Rudd, & Kanyuka, 2017; Motteram et al., 2009)	PF05630	GO:0008150; GO:0003674; GO:0005575
	Zt09_chr_2_00129	Hypothetical protein, secreted phospholipase A2 precursor	PF06951	GO:0004623; GO:0005509; GO:0005576; GO:0016042

Summary of core Z. tritici biotrophic effector candidate genes that were identified based on their specific expression profiles within the Z. tritici core transcriptional program during wheat infection. Functional annotation, PFAM, and GO term information from Grandaubert et al. (2015).