Table 3.

Zymoseptoria tritici core necrotrophic effector candidate genes

Criteria	Significantly up‐regulated at stage C
	Low/no expression during stage A and B
Expression profiles
Putative functions	Facilitate transition from biotrophy to necrotrophy
	Induction of necrosis
Candidate genes	35 (Supporting Information Figure S14)
Candidates encoding hypothetical proteins	24 (Supporting Information Table S16)
Candidates with predicted function	Gene	Functional prediction	PFAM domains	GO terms associated
	Zt09_chr_3_00584	PCWDE, similar to alpha‐1, Glycosyl transferases group 1	PF00128; PF00534; PF08323	GO:0003824; GO:0043169; GO:0005975; GO:0009058
	Zt09_chr_9_00308	PCWDE, similar to carbohydrate‐binding module family 63 protein, expansin‐like	—	GO:0008150; GO:0003674; GO:0005575
	Zt09_chr_3_01063	PCWDE, similar to pectate lyase	PF00544	GO:0008150; GO:0003674; GO:0005575
	Zt09_chr_12_00112	cutinase‐like protein	PF01083	GO:0050525; GO:0016787; GO:0005576; GO:0008152
	Zt09_chr_2_00663	similar to Chain A, cutinase‐like protein	PF01083	GO:0050525; GO:0016787; GO:0005576; GO:0008152
	Zt09_chr_2_01151	PCWDE, similar to acetyl xylan esterase,	PF01083	GO:0016787; GO:0008152
	Zt09_chr_6_00446	PCWDE,similar to acetyl xylan esterase	PF01083	GO:0016787; GO:0008152
	Zt09_chr_10_00107	PCWDE, similar to glycoside hydrolase family 12 protein	PF01670	GO:0008810; GO:0004553; GO:0000272
	Zt09_chr_2_01205	PCWDE, similar to putative extracellular cellulase CelA/allergen Asp F7‐like protein	PF03330	GO:0008150; GO:0003674; GO:0005575
	Zt09_chr_6_00626	similar to metalloprotease	PF05572	GO:0008237
	Zt09_chr_9_00038	similar to hydrophobin	PF06766	GO:0005576

PCWDE: putative plant cell wall‐degrading enzyme.

Summary of core Z. tritici necrotrophic effector candidate genes that were identified based on their specific expression profiles within the Z. tritici core transcriptional program during wheat infection. Functional annotation, PFAM, and GO term information from Grandaubert et al. (2015).