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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Cancer Discov. 2018 Jan 4;8(3):288–303. doi: 10.1158/2159-8290.CD-16-1406

Figure 1.

Figure 1.

(A) Single cell RNA-seq data showing expression of final selected genes in WBCs and single prostate CTCs isolated from patients with metastatic prostate cancer (data from GEO GSE67980). (B) List of genes contained within each multiplex prostate CTC assay, with FAM/HEX ratio for each probe listed below. Genes in green font are androgen-responsive, genes in red font are androgen-repressed, and genes in blue font are androgen-independent. (C) Graph of d-CTC assay signal for varying numbers of LNCaP cells micromanipulated into healthy donor whole blood and processed using the CTC-iChip. (D) Top panel: multi-class support vector machine (SVM) classifier model to automatically classify positive droplet signals. Lower 3 panels: representative multiplex ddPCR expression signal in CTCs from a metastatic prostate cancer patient, a localized prostate cancer patient, and a healthy donor.