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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Placenta. 2018 Aug 20;70:7–14. doi: 10.1016/j.placenta.2018.08.004

Figure 1. RNA interference of FLT1 or KDR is specific.

Figure 1.

(A) Real-time PCR demonstrated more than 80% ablation of FLT1 mRNA expression after transfection with siFLT1 (p<0.01), with no effect on KDR expression. (B and C) A representative western blot with graphical representation also confirmed specificity of FLT1 siRNA (p<0.0001). (D) KDR mRNA expression was decreased by approximately 90% after KDR knock-down (p<0.01), with no effect on FLT1 expression. (E and F) A representative western blot with graphical representation after transfection with KDR siRNA also confirmed ablation of KDR expression with no effect on FLT1 expression (p<0.0001). Mock denotes cells exposed solely to exposure to the lipid.