Fig 1. Overview of the methods used in this study to prioritise compounds likely to ameliorate ageing in humans.

A) STITCH chemicals were mapped into DrugBank drugs using the UniChem resource programmatically. B) The significance of the drug-ageing inference was calculated using a Fisher’s exact test, which calculates the probability that the overlap between two samples (ageing-related genes and drug targets) drawn from the same universe is due to chance. This comparison was made at different biological levels. C) Diagram of the procedure to expand the “gene” information into multiple biological levels. Ageing-related genes were mapped to other levels using an enrichment analysis, while the drugs’ targets were cross-referenced with the list of genes defining each annotation.