Table 2. Phenotypic traits for differentiation between O. quorumnocens sp. nov. and the related Ochrobactrum spp. type strains.

Trait	1A	2	3	4	5	6
Carbon source utilization
Citric acid	-	+	+	+	+	-
d-maltose	-	+	+	-	-	-
Sucrose	-	+	+	-	-	-
l-rhamnose	+	+	+	+	+	-
l-histidine	+	+/-	+	+	+	-
Pectin	-	+	+	-	-	-
Glucoic acid	+/-	+	+	+	-	+/-
Bromo-succinic acid	+	+	+/-	-	+	+/-
Other
Reduction of nitrates to nitrites	-	-	-	+	+	-
Urease	-	-	-	-	-	+
Inactivation of C6-HSL	+	+	-	+	+	+
Growth at 37°C in LB	good	moderate	weak	good	very good	moderate
Motility	+	+	+	+	+/-B	-

^A 1—O. quorumnocens A44^T, 2—O. pituitosum CCUG 50899^T, 3—O. rhizosphaerae PR17^T, 4—O. grignonense OgA9a^T, 5—O. pseudogrignonense CCUG 30717^T, 6—O. thiophenivorans DSM 7216^T

^B motile in the presence of amino acids, immotile in the absence of amino acids in minimal medium with glycerol as a sole carbon source (details in Supplementary data, S3 Table)

All strains were non-hemolytic on Columbia blood agar (BTL, Poland). Following 19 h of incubation of GEN III plates at 28°C, all strains were negative for the utilization of d-cellobiose, d-raffinose, α-d-lactose, d-melibiose, fusidic acid, d-serine, minocyclin, p-hydroxy-phenylacetic acid and did not show growth in the presence of 8% NaCl and at pH 5. In contrast, all strains metabolized α-d-glucose, d-mannose, d-fructose, d-galactose, d-fucose, L-fucose, 1% sodium lactate, d-arabitol, L-alanine, L-glutamic acid, d-galacturonic acid, d-galactonic acid lactone, d-glucuronic acid, and grew in the presence of rifamycin SV, vancomycin, troleandomycin, guanidine HCl, tetrazolium violet, tetrazolium blue, L-lactic acid, L-malic acid, lithium chloride, potassium tellurite, acetoacetic acid, acetic acid, aztreonam.