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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Sci Transl Med. 2018 Aug 22;10(455):eaaq0693. doi: 10.1126/scitranslmed.aaq0693

Fig. 3. HDM-treated lta4h−/− mice exhibit PGP accumulation but comparable neutrophil infiltrate relative to littermate controls.

Fig. 3.

Wild-type and lta4h−/− mice were administered HDM or PBS intranasally three times per week for 3 weeks, and BALF and lung tissue were collected 24 hours after the final HDM/PBS exposure. Amounts of MMP-9 in BALF were assessed by ELISA (A) and prolylendopeptidase by Western blot (B). The concentration of PGP in BALF was determined by mass spectrometry (C). BALF was incubated with exogenous PGP and degradation assessed after 2 hours by mass spectrometry (D) or release of free proline (E). The number of neutrophils recruited into the lung tissue (F) and airways (G) was determined by flow cytometry. The concentration of MPO (H) in the BALF was determined by ELISA. Figures present combined data from two independent experiments with four to six mice per group in each experiment. Results depicted as means ± SEM. ***P < 0.001, using Mann-Whitney statistical test.