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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: ACS Chem Neurosci. 2017 Aug 18;8(11):2468–2476. doi: 10.1021/acschemneuro.7b00239

Figure 1. Sobetiramides are substrates of FAAH.

Figure 1.

(A) Chemical structures of thyroid hormone (T3), sobetirome, two endogenous substrates of FAAH (AEA and OEA), and novel FAAH-targeted sobetirome amide (sobetiramide) prodrugs. (B) Using cell homogenate overexpressing human FAAH, Michaelis-Menten curves were produced for both Sob-AM1 and Sob-AM2 compared with the endogenous FAAH substrate AEA. Hydrolysis reactions (n=3) were monitored by quantifying product formation by LC-MS/MS. A summary of Vmax and Km values can be found in Table 1. For each substrate concentration, reaction time and protein levels were adjusted to maintain <10% substrate conversion. Data represent mean ± SEM