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. Author manuscript; available in PMC: 2019 Jan 22.
Published in final edited form as: Cancer Immunol Res. 2018 Apr 13;6(6):645–657. doi: 10.1158/2326-6066.CIR-17-0554

Figure 1. Th17 cell infiltration in early and late human NSCLC.

Figure 1

(A) Representative intracellular, and (B) cumulative (n = 15) staining for paired analysis of lung CD4+ T cells expressing IL17A (Th17) and IFNγ (Th1) isolated from tumor-free adjacent tissue (T-FAT) and tumor infiltrated lymphocytes (TIL) in early stage (I–II) surgical samples (n = 6). (C) PD-1 cell surface expression in the same cell population (n = 6). (D) Representative intracellular, and (E) cumulative staining for paired analysis of tumor-free (T-FLN) and tumor-positive (T-PLN) lymphocytes expressing IL17A (Th17) and IFNγ (Th1) in sentinel lymph node in late stage (III–IV) NSCLC. (F) Representative PD-1 expression in CD4+ T cells and (G) cumulative (n = 12) in the same cell population. Correlation between PD-1+ CD4+ T cells and Th17 cells in (H) T-FLN (n = 21) or (I) T-PLN samples (n = 14). **P < 0.01, * P < 0.05 as determined by the Paired Student’s t-test. p and r value were obtained by linear regression model. Data are mean ± SEM. Please also see Supplementary Fig. S1.