Figure 4. Global loss of IL17a in Pts4^d/d mice reduces primary tumor latency and increases metastasis.

(A) Representative images of microCT scan mid thorax in 6 month-old Pts4^d/d, and Pts4^d/dIl17a^−/− mice. Red circle identifies detection of tumor in the right lobe. (B) Representative H&E of the lungs (top two panels), and 4X magnifications (depicted in rectangles) detect epithelial hyperplasia in Pts4^d/d mice, and adenosquamous tumor in Pts4^d/dIl17a^−/− mice. Bottom panel show representative of stomach H&E, shows no evidence of metastasis in Pts4^d/d mice, while tumor invasion is localized to stomach parenchyma in Pts4^d/dIl17a^−/− mice (arrow). scale bar = 200 μm. (C) Primary adenosquamous lung cancer detection (%) in Pts4^d/d and Pts4^d/dIl17a^−/− mice. (D) Cumulative analyses of the frequency of lung tumor and stomach metastases of Pts4^d/d and Pts4^d/dIl17a^−/− mice at indicated ages. (E) Representative lung tissue immumohistochemical analysis of Sox2, TTF1, and CK7 in Pts4^d/d and Pts4^d/dIl17a^−/− mice. *** P < 0.001 as determined by the paired Student’s t-test. Please also see Supplementary Fig. S4.