Figure 5. Comprehensive immunophenotype in 7-month old Pts4^d/dIl17a^−/− mice.

Representative flow gating, and ICC (A) and cumulative analysis (B) for lung IFNγ expressing CD8⁺ cells(CTLs), CD4⁺ (Th1), foxp3⁺ expressing (Tregs), and PD-1 expression in 7-month old age matched mice: WT (Pts4^f/), Pts4^d/d, and Pts4^d/dIl17a^−/− (data pooled from two independent experiments; n = 9 per group) (C) Representative flow gating and relative abundance of lung CD103⁺ DCs in CD45⁺CD11c⁺CD11b⁻ lung cells and PD-L1expression in CD11b⁺ DCs in the same groups of mice. (D) Representative histogram and (E) cumulative relative abundance of CD103 expression, and MFI, and PD-L1 expression in CD11c⁺CD11b⁻ DC subset described in panel C. (n = 5 or 6 per group) (F) Representative histogram and (G) cumulative expression of CD206 on F4/80 tumor-associated macrophages (TAM) in whole lung single cells homogenates in the indicated groups of mice. (H) Quantitative expression of Arg1, Vegf and Il10 gene using qPCR in BAL cells isolated from the same groups of mice. (n = 5 or 6 per group). Data are mean ± SEM and representative of three independent experiments; *** P < 0.001, ** P < 0.01, * P < 0.05 as determined by the one-way ANOVA and Bonferroni’s Multiple Comparison test. Please also see Supplementary Figs. S5, S6, S7, S8, and S9.