Figure 7. Adoptive transfer of IL17a sufficient CD4⁺ T cells reverses decreased tumor latency and metastasis in Pts4^d/dIl17a^−/− mice.

15 × 10⁶ wild type CD4⁺ T cells were adoptively transferred to Pts4^d/dIl17a^−/− mice at 2.5 and 5 months of age. (A) Representative H&E staining of lung tumors in the indicated groups of mice (inset 4× magnification). scale bar = 100 μm. (B) Frequency of lung tumor and stomach metastases in Pts4^d/d and Pts4^d/dIl17a^−/−, Pts4^d/dIl17a^−/− (+WT CD4) mice at 7 and 8 months. (C) Relative abundance of Th17 cells, (D) ICC detection of IFNγ and IL17A in lung CD4⁺ and CD8⁺ T cells (CTLs) respectively. (E) Relative expression of CD103 in lung DCs (CD11c⁺CD11b⁻) in the same groups of mice. (F) ICC detection of IFNγ in lung CD8⁺ T cells (CTLs) in mediastinal lymph nodes in the same groups of mice at 7 months of age. (G) Relative expression of CTLA in CD4⁺ T cells in mediastinal lymph nodes of the same groups of mice. Data are mean ± SEM and representative of two independent experiments (C – G); ** P < 0.01, * P < 0.05 as determined by the Chi-square test (B) or the one-way ANOVA and Bonferroni’s Multiple Comparison test (C-G).